Biology Talk Biology Talk    FAQ   Search   Memberlist   Usergroups   Register   Profile   Log in to check your private messages   Log in  Cell senescence          Biology Talk Forum Index -> Biotechnology View previous topic :: View next topic   Author Message poojamajmudar@gmail.com Guest Posted: Tue Apr 24, 2007 10:05 pm    Post subject: Cell senescence Hello all, I am experiencing a very wierd problem. I am trying to grow primary cultures of endothelial cells. As of now I am culturing mouse heart endothelial cells. The cells in the beginning look alright, having typical cobblestone appearance, but over a period of a day or teo, those cell flatten out and I think they are becoming senescent. I am growing the cells in highglucose DMEM supplemented with 10% FBS at 37deg C. I do not understand why are the cells doing this? Thank you, Pooja Back to top   Ads Advertising Sponsor Display posts from previous: All Posts1 Day7 Days2 Weeks1 Month3 Months6 Months1 Year Oldest FirstNewest First         Biology Talk Forum Index -> Biotechnology All times are GMT Page 1 of 1   Jump to: Select a forum Biology Talk----------------Biology Talk-- MicrobiologyEvolutionPsychobiologyBiotechnology  You cannot post new topics in this forum You cannot reply to topics in this forum You cannot edit your posts in this forum You cannot delete your posts in this forum You cannot vote in polls in this forum 109 Attacks blocked Powered by phpBB © 2001, 2005 phpBB Group